产品名称：Epithelial Antigen （Ep-CAM）

详细介绍：

Name: Epithelial Antigen （Ep-CAM） （C-term） Rabbit Antibody PDF of Datasheet

Clone ID: E144
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1144-1
Size: 100ul
Price: $ 235

Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse, Rat

Mol. Weight: 39 kDa
UniProt ID: P16422

Description: Epithelial cell adhesion molecule （Ep-CAM） is a monomeric membrane glycoprotein that is expressed on virtually all epithelial cell membranes and on a variety of epithelial carcinomas （1-3）. Ep-CAM is overexpressed in a variety of human carcinomas and is, therefore, a potential therapeutic target for human solid tumors （4）. Ep-CAM contains an extracellular domain with two epidermal growth factor-like repeats, followed by a cysteine-poor region, which are necessary for the adhesion properties of the molecule （5）.

Specificity: A synthetic peptide corresponding to residues in C-terminus （cytoplasmic domain） of human Ep-CAM was used as immunogen.

Recommended Dilutions:
WB: 1:2,500
IHC: 1:100-1:250
ICC: 1:100

Protocols for using Epitomics Rabbit Monoclonal Antibodies

A. Western blot analysis on A431 cell lysate using anti-Epithelial Antigen （Ep-CAM） （C-term） RabMAb （cat. #1144-1）; dilution 1:2,500.	B. Immunohistochemical analysis of paraffin-embedded stomach carcinoma using anti-Epithelial Antigen （Ep-CAM） （C-term） RabMAb （cat. #1144-1）.

References:

1.	Tomita, Y., et al. Molecular identification of a human carcinoma-associated glycoprotein antigen recognized by mouse monoclonal antibody FU-MK-1. J. Cancer Res. 91: 231–238 （2000）.
2.	Trebak, M., et al. Oligomeric state of the colon carcinoma-associated glycoprotein GA733-2 （Ep-CAM/EGP40） and its role in GA733-mediated homotypic cell-cell adhesion. J. Biol. Chem. 276: 2299–2309 （2001）.
3.	Simon, B., et al. Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc Natl Acad Sci U S A. 87: 2755–9 （1990）.
4.	Szala, S., et al. Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2. Proc Natl Acad Sci U S A. 87: 3542–6 （1990）.
5.	Balzar, M., et al. Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol. Cell. Biol. 21: 2570–2580 （2001）.

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