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Name: NGF-β Rabbit Antibody PDF of Datasheet |
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Clone ID:
EP1320YAntibody Type:
Rabbit Monoclonal IgG Catalog#:
1895-1Size:
100ulPrice:
$ 235Recommended Applications:
WB, IHC, ICCSpecies Reactivity:
Human, Mouse, RatMol. Weight:
32 kDaUniProt ID:
P01138Description: Nerve growth factor (NGF) stimulates division and differentiation of sympathetic and embryonic sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurons (1). NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. While NGF is found in almost all vertebrates, most research has focused on murine NGF, as the mouse male submaxillary gland contains higher levels of this polypeptide than other tissues (2).
Specificity: A synthetic peptide corresponding to residues near the C-terminus of human NGF-β was used as an immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:50 – 100
ICC: 1:100 -250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on fetal thyroid tissue using anti-NGF-β RabMAb (cat. # 1895-1), dilution 1:500. |
B. Immunohistochemical staining of paraffin-embedded human brain using anti-NGF-β RabMAb (cat. #1895-1). |
References:
| 1. |
Levi-Montalcini R. Nature 237(4819):1154-62, 1997. |
| 2. |
Ullrich A, et al. Nature 303(5920):821-5, 1983 |
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